Interdisciplinary Studies on Applied Science

Research Article

Phytochemical Investigation And Antibacterial Activity Of Dried Lime Citrus Aurantifulia

  • By Anfal Izaldeen Al Kateeb, Dhifaf Jabbar Shamran, Emaduldeen Hatem Abed - 21 Jul 2024
  • Interdisciplinary Studies on Applied Science, Volume: 1(2024), Issue: 1(July-December), Pages: 12 - 19
  • https://doi.org/10.58613/isas112
  • Received: June 9, 2024; Accepted: June 30, 2024; Published: July 21, 2024

Abstract

To continue the efforts of screening natural products to obtain more active drugs and safer to use, this study established to screening the phytochemical compounds on dried lime Citrus aurantifulia and evaluate the antibacterial activity of its water extract on several bacteria species. Via GC-MS analysis, the most phytochemicals were found in water extract of C. aurantifulia, dl-Limonene (34.05%), alpha. -Terpineol (13.45%), Terpinene gamma (4.56%), Alpha.-terpinolene (4.56%), Pinene beta (3.88%), endo-Borneol (3.26%), beta.-Bisabolene (3.31%). The Kirby-Bauer disk diffusion susceptibility test was utilized to determine the antibacterial activity of the extract at various concentration (200, 100, 50, 25, 12.5, and 6.25 mg/ml)., it’s found to inhibited the bacterial growth as dose depending manner, where the inhibition zone were (25, 25, 19, 25, 26 and 23mm) against E. faecalis, St. aureus, B. cereus, E.coli, K. pneumoniae and P. aeruginosa, respectively. In conclusion, the dried lime has a high potential to be used as antibacterial drug in many fields.