Research Article
The discrimination between clinical samples otitis extern and aspergillosis by using random primer OPM-20 and comparative with other random primers
- By Nawras Kadhim Rassin, Marwa Kalid Razaq, Ola Hamed Muhmoud, Asmaa Nafea Badri, Neamet Jameel Abdulbaqi - 27 Aug 2024
- Journal of Biomedicine and Biosensors, Volume: 4, Issue: 3, Pages: 33 - 38
- https://doi.org/10.58613/jbb434
- Received: 19 May 2024; Accepted: 05 August 2024; Published: 27 August 2024
Abstract
Where genetic divergence and distance between showed that the DNA pure showed OPM-20 primers consisting of 10 bases with high energy efficiency by 19.1 amount 16 of the number of bundles total and the number 8 of unique bundles and the amount compared OPD- 20 primers with the amount energy 1.33 of 30 the number of bundles total and1 unique bundles. Comparing other primers of RAPD-PCR such as OPE-16, OPN-07, OPQ-1, and OPL-05 primers, it appears that they can distinguish random isolates. Still, they are less efficient in distinguishing those isolates from some of them than OPM-2. The use of a dendrogram showed how the isolates were close to aspergillosis and the internal otitis depended on the genetic distance between those two isolates was close. The third isolate resulting from contamination covers and furnishings of hospitalized patients resulting from the spread of fungal spores of captive patients was farthest away, and the difference was simple in its genetic arrangement.