Journal of Biomedicine and Biosensors

Research Article

Association between IL-2 Levels and IL-2-rs2069762 Gene Polymorphism in Patients with Hashimoto's Disease

  • By Ali S Shakir - 31 Jan 2024
  • Journal of Biomedicine and Biosensors, Volume: 4, Issue: 1, Pages: 1 - 13
  • https://doi.org/10.58613/jbb411
  • Received: January 1, 2024; Accepted: January 20, 2024; Published: January 31, 2024

Abstract

Background: Hashimoto’s thyroiditis (HT) is one of the most prevalent organ-specific autoimmune diseases worldwide at any age, and is induced by loss of immune tolerance of thyroid gland. Over the past few decades, its occurrence has increased. It is a classic example of an organ-specific autoimmune disease, wherein in genetically predisposed individuals, environmental and existential circumstances set off the immune system's attack against thyroid antigens.
Aim: The present study aims to evaluate the role of IL-2 genes polymorphisms and their serum levels in the predisposition to HT.
Methods: The research project included 60 patients with Hashimoto's thyroiditis disease, ages ranging from 21 to 65, who hospital admission between March and August of 2023. The other group consisted of 60 people who appeared to be health individuals. Five milliliters of blood were taken, two milliliters of every specimen being used for the PCR amplification and IL-2 (rs2069762) gene detection (RFLP-PCR) method. The remaining (3ml) for IL-2 ELISA (Mabtech USA) Kit test.
Results: The IL-2 genotype did not statistically correlate with a higher risk of HT (P= 0.352). On the other hand, those with the TG genotype had greater serum levels of IL-2 than those with the TT or GG genotype (P= 0.172). HT patients' serum IL-2 levels were statistically greater than those of the healthy control group. Conclusions: a insignificant correlation between IL-2 genotype and serum IL-2 concentration in patients with HT.